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Growth hormone-induced insulin resistance in human subjects involves reduced pyruvate dehydrogenase activity

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Freshly isolated islets were transferred into Falcon 35 mm sterile dishes and cultured o/n in Roswell Park Memorial Institute medium (RPMI-1640) containing 5.6 mM glucose supplemented with 10% FBS, 100 U/mL penicillin, 100 g/mL streptomycin, 10 mM HEPES, 2 mM L-glutamine, 1 mM sodium pyruvate and 50 M -mercaptoethanol

Animal model studies consistently demonstrated that semaglutide can significantly alter gut microbiota, suggesting that part of semaglutides metabolic benefit may derive from its capacity to modulate inflammation via gut-immune-microbiota interactions and to correct dysbiosis induced by high-fat diets [82, 84]