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Immunoassay Tear fluid was extracted from half of the remaining strip by agitating small cut pieces of these strips in 100 L PBS, Tween20 0.05% and cOmplete Protease Inhibitor Cocktail (Roche, Basel, Switzerland) at 4 C for 1.5 h 28
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Plant-based sources: Seeds, pulses, legumes (for those on plant-based diets, protein intake should be carefully managed to ensure sufficient amino acid diversity)
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